Composite
C9

Part:BBa_K1366108

Designed by: Eduardo Cepeda Cañedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)


Apoptin and survivin siRNA production (C9)

This construct will be used to characterize the transfection of two effectors of death (Survivin siRNA and apoptin) in mammalian cells mediated by bacteriophages. The survivin siRNA is expressed under an hTERT promoter, specific for cancerous cells lines. The apoptin is expressed under a CMV promoter because it is extremely specific, so it wouldn't harm the normal cells. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1407
    Illegal BsaI site found at 2402


[edit]
Categories
Parameters
None